Unleash The Fun With Royal Panda’s Online Casino Games – After years of disappointment, staff at Edinburgh Zoo were hoping a baby panda would be born later this month. But is captive breeding really the way to save the species?
Around 5pm on March 25, on a cold and wet Wednesday earlier this year, Edinburgh Zoo’s female giant panda Tian Tian moved from a wooden platform in her outdoor enclosure and began to bleat. Born at the Beijing Zoo in 2003, Tian Tian has been a hit with visitors since arriving in the UK with male panda Yang Guang at the zoo in December 2011. Yang Guang, whose name means “sun”, may be bigger and more. Apparently, he’s a kinder creature, but Tian Tian (“My Beloved”) is a panda with more edges, wit, and more impulsiveness.
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These are extraordinary features. Pandas are vegan, slow-metabolic bears. They feed almost exclusively on bamboo, which they digest poorly. They do everything in their power to avoid unnecessary effort. If you give Yang Guang a ball, he will most likely see if he can eat it and then let go. Tian Tian, on the other hand, is known to skip balls and perform forward flips. Sometimes she hangs from the bars on top of her hideout – a position her keepers call “ninja panda” – just like that, for hell. Not everything is cool. Tian Tian abused the bouncers, occasionally slapping her massive claws at passing veterans. “She definitely had a mind of her own,” said Alison McClain, head keeper of pandas at Edinburgh Zoo, who seems to love pandas very much for exactly this reason. “You have to be very, very careful.”
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That afternoon, as they prepared to go out for the day, the zoo’s team of pandas kept a close eye on Tian Tian. As she stood and wandered through the pale green barrier separating her cover from Yang Guang, McClain, who sat in his office a few hundred yards away, followed her on one of the 16 cameras, watching the bears day and night. The end of March marks the middle of the short, delicate and awkward panda breeding season. Female pandas only ovulate once a year. The ideal window for them to mate – “drop zone” in zoo parlance – is about 24 hours. Depending on the vagaries of climate, diet, and bear, the build-up to this point may be obvious and take weeks, or it may come suddenly without any warning. “The signs can be very subtle,” Simon Girling, a veterinarian at Edinburgh Zoo, told me. “We’re always worried about missing a window.”
On the screen, McClane watched as Tiantian approached Grid and continued their conversations. Pandas that have evolved to live solitary lives are kept in separate enclosures in zoos to prevent them from killing each other. The only exception is during breeding season when bears are called upon to communicate and eventually, euphemistically, “introduced”. Seeing Tian Tian through the bars, Yang Guang, who had spent the afternoon sitting at the top of the cave eating, called back. “We thought it was very good,” said McClain. “Very interactive, very cute.”
McClain was cautious. Since the pandas arrived, the team at Edinburgh Zoo have already tried to breed the bears three times – with great fanfare and public attention – and each attempt has ended in disappointment. After an exhaustive review of these trials in late 2014, this year’s season brought with it a sense of added pressure. But bouncers have also invented one or two new tricks. A few weeks ago, McClain painted Paul Long Hui, an imposing male panda at Schönbrunn Zoo in Vienna, in the Yang Guang and Tian Tian enclosures, to spice up the atmosphere with competition and opportunity. “She spent a lot of time sniffing and watching what was going on,” McClain said. He came out and said, “Wow! “It was everywhere.”
However, as in previous years, most of the matchmaker’s job was to closely monitor Tian Tian’s bamboo consumption, behavior, and daily vulva photos of Tian Tian – to make sure everyone was ready for the big moment. An important part of this monitoring is the analysis of hormones in Tian Tian’s urine. Since mid-March, McClain and her team have been trying to collect up to four samples a day, but it’s a challenge. Females that come into estrus have evolved to urinate in puddles and streams to warn potential mates, and while Tian Tian has been trained to urinate on command, she often refuses to obey. “You often say, ‘I’m sticking to this,’” McClain said. Syringe Guardians rush to collect precious drops from the ground when you’re not looking.
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Urine samples are taken by bus or taxi to Forbes Howey, an endocrinologist at the University of Edinburgh, but final interpretation is carried out by Iain Valentine, panda project manager at Edinburgh Zoo. A tall, toothy, muscular man, Valentine is a maverick and misfit from Edinburgh, the one who first dreamed of bringing pandas to Scotland – the northernmost place where animals live – some ten years ago. Since then, he has been the evangelist and architect of the zoo’s effort to breed Britain’s first baby panda. Every spring, Valentine, 51, an expert on the capercaillie, the great woodland bird, spends weeks stumbling over fluctuating levels of estrogen and progesterone in pandas, as well as many other proteins, looking for telltale signs of approaching ovulation – “twin peaks.” and “The Big Fall” – and we’re waiting for the next set of results from the lab.
By the afternoon of March 25, Valentine was convinced that Tian Tian had reached her “crossover” – the threshold where her estrogen levels exceed those of progesterone – 12 days earlier and that she had ovulated in two or three days. The warning scale is important. Because pandas are so rare and the chances of them breeding are negligible, it is standard practice in zoos to artificially inseminate females, as well as attempting to mate naturally. For scientific support and political relations (all pandas are officially on loan from the Chinese government), Valentine has already hired two experts from the CCRCCGP – China’s main panda research center – at the Holiday Inn adjacent to the zoo, and has booked a team of reproductive biologists from Germany to fly the next day to prepare for in vitro fertilization. The Germans, led by Professor Thomas Hildebrandt of the Leibniz Institute for Animal and Wildlife Research, artificially inseminated Tian Tian over the past two years.
Watch Valentine Tian Tian through webcam. He noticed a slight drop in estrogen in a urine sample taken that morning at 8:30 am, but didn’t care too much. The team took a second sample at 9:50 a.m. but chose not to send it, waiting for the results with the afternoon sample, which was taken at 2:30 p.m. “We thought nothing big was going to happen,” said Valentine. Not expecting any new data until late in the evening, and quietly encouraged by the images of Tian Tian and Yang Guang speeding through the grid, the panda team returned home.

Valentine lives in Newburgh, a village on the banks of the River Tay about an hour north of the zoo. He was getting ready for dinner when, at 7:08 p.m., he received an enthusiastic letter from his endocrinologist Howie. “The scores are even,” she said. “Recidivism!” Tests showed that Tian Tian’s estrogen levels suddenly dropped in the short period between the two samples that morning. She almost certainly ovulated, and that was almost 12 hours ago. Tian Tian was in the relegation zone. “Sneaky minx,” Valentine replied in a text message. The clock ticked, and his mind began to race with decisions and difficulties—all the people he had to summon and bring together. “The Chinese are at the Holiday Inn. home veterinarians. The guards are in the house, he said to himself. “Team Berlin is in Berlin.”
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His first contact was Zhaoyuan Li, a Chinese field biologist based in Scotland who had been serving at the zoo as a translator and liaison with Chinese scientists and bureaucrats since 2008. Li was at a hotel with two Chinese scientists, and Valentine wanted them both. Test results. Then call Girling, the head vet. McClain got a call from one of her guards while she was at the supermarket. She put the groceries back on the shelves and ran to the car. The Germans tried to change flights.
The first giant panda in captivity, Ming Ming, was born on October 9 at the Beijing Zoo
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